ABSTRACT Dopamine, a crucial neurotransmitter, plays a central role in the pathogenesis of neurological and psychiatric disorders. Accurate and precise measurement of dopamine levels is critical for the early diagnosis and monitoring of these conditions. For the first time, a Zr‐MOF‐based colorimetric sensor system exhibiting peroxidase‐like activity was developed for the detection of dopamine. In the presence of H2O2, the Zr‐MOF nanozyme catalyzes the oxidation of the TMB substrate, producing a blue‐colored oxidized product. Upon the addition of dopamine, H 2 O 2 is consumed, which inhibits the nanozyme‐mediated oxidation of TMB and consequently reduces the optical signal. Systematic optimization studies revealed that the most efficient catalytic activity was achieved with 100 µL of Zr‐MOF suspension (1000 µg/mL), a 30‐min reaction time, and ambient temperature (25 °C). Selectivity studies demonstrated that compounds commonly found in biological environments, such as potassium chloride (KCl), sodium chloride (NaCl), aspartic acid, and urea, resulted in recoveries of approximately 95%. Serum matrix effect tests yielded a recovery of 96% in an artificial serum environment, and analysis of pharmaceutical dopamine ampoules achieved a recovery of 99.6%. However, the sensor exhibited limited sensitivity at low analyte concentrations, indicating the need for performance enhancement to achieve lower detection limits. Overall, the developed sensor provides a simple, selective, and cost‐effective platform for the detection of biologically significant analytes.
Kaya et al. (Sun,) studied this question.