What question did this study set out to answer?

The research aims to evaluate the effects of antigen heterogeneity on the accuracy of a multiplexed urine test for tuberculosis.

March 13, 2026Communications Medicine0 citationsOpen Access

Antigen heterogeneity in the development and clinical validation of a multiplexed urine test for tuberculosis

TDTyler DouganBrigham and Women's Hospital SRShira RothBrigham and Women's Hospital LXLiangxia XieBrigham and Women's Hospital

Key Points

The research aims to evaluate the effects of antigen heterogeneity on the accuracy of a multiplexed urine test for tuberculosis.
Evaluated antibody pairs targeting different epitopes on LAM
Compared assay sensitivity to AlereLAM lateral flow test
Utilized urine samples from HIV-positive individuals
The multiplexed urine test showed higher sensitivity than the AlereLAM test
Adding different antibody pairs did not enhance assay accuracy
Urine samples proved safe and accessible for testing

Abstract

Different antibody pairs detecting different epitopes on LAM report diverging concentrations. We do not find that adding antibody pairs to detect different epitopes on LAM improves the assay's accuracy. Our assay is more sensitive than the existing AlereLAM lateral flow test for TB in HIV-positive individuals, uses safe and accessible urine samples, and represents a step towards an adjunctive diagnostic test to aid clinicians in starting treatment.

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Cite This Study

Dougan et al. (Tue,) studied this question.

synapsesocial.com/papers/69b3ac8102a1e69014cce3e7 https://doi.org/https://doi.org/10.1038/s43856-026-01458-0

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