Traditional mRNA delivery systems typically require several hours to achieve efficient cellular internalization, and non-internalized mRNA payloads undergo degradation over time. In this study, we develop a fast mRNA delivery platform termed magnetic responsive lipid nanoparticles (MrLNPs) for efficient mRNA delivery. The magnetic responsiveness and enhanced cellular uptake of MrLNPs are confirmed using various LNP-based formulations to deliver diverse mRNA payloads to multiple cell types, demonstrating their broad applicability. Notably, a single administration of MrLNPs enabled fast mRNA delivery and protein expression in mice upon noninvasive magnetic stimulation, achieving a more than 33-fold increase in delivery efficiency compared to conventional non-magnetic LNPs. Overall, this work establishes a novel magnetic responsive mRNA-LNP platform capable of fast mRNA delivery both in vitro and in vivo, with potential applications in acute critical illnesses requiring immediate protein expression.
Lin et al. (Thu,) studied this question.