What question did this study set out to answer?

The research aims to clarify how hsa_circ_0081241 contributes to the progression of acute myocardial infarction (AMI).

March 19, 2026Open Access

Hsacirc₀081241 is involved in the progression of acute myocardial infarction through the miR-15b-5p/PHF19 axis

Key Points

The research aims to clarify how hsa_circ_0081241 contributes to the progression of acute myocardial infarction (AMI).
Assessed expression levels of hsa_circ_0081241, miR-15b-5p, and PHF19 using RT-qPCR and western blotting.
Evaluated cell viability and apoptosis with CCK-8 and Annexin V-FITC/PI kits.
Measured pro-inflammatory factors with ELISA and analyzed macrophage polarization.
Investigated oxidative stress through ROS and SOD levels.
Used ROC curves and binary logistic regression to assess clinical values.
Hsa_circ_0081241 promoted M1 macrophage polarization and fibrosis in myocardial fibroblasts.
It increased PHF19 expression by down-regulating miR-15b-5p, leading to oxidative stress in hypoxic conditions.
The combined AUC for hsa_circ_0081241, miR-15b-5p, and PHF19 as AMI predictors was 0.871.

Abstract

Circular RNAs (circRNAs) are believed to play a role in various human diseases, but their involvement in acute myocardial infarction (AMI) remains poorly understood. This study aims to clarify the role of hsacirc₀081241 in AMI and elucidate its mechanism of action. RT-qPCR and western blotting detected the expression of hsacirc₀081241, miR-15b-5p, and PHD finger protein 19 (PHF19). A dual-luciferase reporter assay demonstrated their targeting relationships. Cell viability and apoptosis were assessed using CCK-8 and Annexin V-FITC/PI kits. M1 polarization of human bone marrow macrophages (hBMMs) was evaluated by detecting iNOS and CD68/CD86 expression. ELISA kits measured IL-6 and IL-12 secretion levels. Collagen I/III expression was measured to assess fibrosis. Reactive oxygen species (ROS) and superoxide dismutase (SOD) levels were measured to evaluate oxidative stress damage. The clinical value of the hsacirc₀081241/miR-15b-5p/PHF19 axis was assessed using receiver operating characteristic (ROC) curves and a binary logistic regression model. Hsacirc₀081241 promoted hypoxia-induced functional impairment in human cardiomyocytes (AC16) and mediated M1 polarization of hBMMs, driving fibrosis progression in human myocardial fibroblasts (hMFs). Pro-inflammatory factors secreted by M1 macrophages further aggravated hMF fibrosis. Hsacirc₀081241 promoted PHF19 expression by down-regulating miR-15b-5p, mediating oxidative stress injury in hypoxia/reoxygenation (H/R) -damaged AC16 cells and myocardial tissues of the AMI rat model. Hsacirc₀081241, miR-15b-5p, and PHF19 were independent predictors of AMI, with a combined area under the curve (AUC) of 0. 871. The hsacirc₀081241/miR-15b-5p/PHF19 signaling axis is a potential biomarker for AMI. It may play an important role in myocardial injury caused by hypoxia and inflammatory microenvironments in this disease context.

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