This document is a pre-registered experimental protocol deposited before any sequencing data has been generated. It is locked at the time of Zenodo deposit and may not be modified after that point. The field-arrest model of domestication (Lawson, 2026, doi:10.5281/zenodo.19094935) proposes that the domestication syndrome is produced by EZH2-mediated H3K27me3 deposition at neural crest cell (NCC) migration driver loci during embryogenesis, arresting NCC migration at the juvenile-proximate developmental stage. This is the same molecular mechanism that maintains cancer attractor arrest in triple-negative breast cancer (TNBC), where EZH2 deposits H3K27me3 at FOXA1, GATA3, and ESR1, silencing the luminal identity program. This pre-registration specifies the ChIP-seq experiment that tests the molecular mechanism prediction. Domestic Sus scrofa embryos at gestational day E14--E18 (peak NCC active migration window) are predicted to show significantly elevated H3K27me3 enrichment at the following pre-registered NCC migration driver loci relative to wild boar embryos at the same developmental stage: BMP4, BMP7, WNT1, WNT3A, CXCL12, CXCR4, EDNRB, SOX10, SNAI2. Primary success criterion: minimum 4 of 9 target loci reach enrichment ratio ≥ 1.5 (domestic/wild boar) at q < 0.05 (FDR-corrected), with negative control loci (GAPDH, ACTB, MYF5, HBB) showing no differential enrichment, and RNA-seq concordance confirming functional silencing at ≥ 3 confirmed loci. The genetic model predicts no differential H3K27me3 at NCC loci between domestic and wild boar embryos at equivalent developmental stages. The field-arrest model predicts specific locus-directed enrichment. The models are distinguished by this experiment. P9 is the gateway experiment for P11 (tazemetostat in pregnant domestic sow, shifting F1 morphology toward wild-type). P11 is not justified until P9 confirms EZH2 as the arrest maintenance enzyme. If P9 confirms, the same drug (tazemetostat) that de-arrests cancer cells by removing H3K27me3 at identity loci is predicted to de-arrest domestic pig embryos by removing H3K27me3 at NCC migration loci. Same enzyme. Same mark. Same drug. Different scale. Pre-specified failure diagnoses (F1--F4) are included. A null result is informative and will be published in full. Theory paper: doi:10.5281/zenodo.19094935 P7 pre-registration: doi:10.5281/zenodo.19096128 Repository: github.com/Eric-Robert-Lawson/attractor-oncology
Eric Robert Lawson (Wed,) studied this question.