Introduction:: Cryopreservation has become a critical technique across various domains, including clinical care, biotechnology, and plant and animal biology. It enables the longterm storage of cells, tissues, and biological products at ultra-low temperatures, preserving their viability and functionality. Cryoprotective Agents (CPAs) are essential for preventing freezingrelated cellular injury, while lyoprotectants play a key role in stabilising biological materials during freeze-drying. Methods:: A comprehensive literature review was conducted focusing on the classification, mechanisms, and applications of cryoprotectants and lyoprotectants. Emphasis was placed on understanding their roles in inhibiting ice crystal formation, minimizing cellular damage, and improving formulation stability. Key concerns such as CPA toxicity and strategies to reduce it, as well as the role of lyoprotectants in nanoformulations and biopharmaceuticals, were explored. Result:: Widely used CPAs like glycerol, propylene glycol, and dimethyl sulfoxide (DMSO) effectively reduce intracellular ice formation and cellular dehydration. However, their use is often limited by cytotoxicity. In contrast, lyoprotectants such as sugars and polymers have demonstrated significant efficacy in stabilising biomolecules and nanoparticles during lyophilisation, enhancing product shelf life and therapeutic effectiveness. Discussion:: The dual approach of cryopreservation and lyophilisation using protective agents has revolutionised drug development, organ preservation, and biologics formulation. Despite their benefits, toxicity and optimisation challenges remain areas of active research. Conclusion:: Cryoprotectant and lyoprotectant are indispensable in modern pharmaceutical and biomedical applications. Their continued innovation is essential for advancing therapeutic stability, organ preservation, and the success of transplantation and regenerative medicine.
Piyush et al. (Mon,) studied this question.