What question did this study set out to answer?

This research aims to compare different methods of testing ceftobiprole susceptibility in Staphylococcus aureus isolates.

March 25, 2026Open Access

Comparison of ceftobiprole 5 μg disk diffusion, MIC test strip, and broth microdilution for susceptibility testing of Staphylococcus aureus clinical isolates

Key Points

This research aims to compare different methods of testing ceftobiprole susceptibility in Staphylococcus aureus isolates.
Systematic comparison of disk diffusion, MIC test strip, and broth microdilution methods.
Evaluation based on EUCAST and FDA interpretive criteria.
Testing both methicillin-susceptible and resistant isolates.
Demonstrated variance in test performance between methods for MSSA and MRSA.
Provided recommendations for accurate reporting of susceptibility results based on method used.

Abstract

Ceftobiprole offers a β-lactam option for Staphylococcus aureus, including methicillin-resistant Staphylococcus aureus (MRSA); however, its susceptibility testing remains insufficiently examined across both European Committee on Antimicrobial Susceptibility Testing (EUCAST) and Food and Drug Administration (FDA) interpretive criteria. We systematically compared MIC Test Strip (MTS) and disk diffusion (DD) with broth microdilution (BMD), demonstrating breakpoint-specific performance for methicillin-susceptible Staphylococcus aureus (MSSA) and MRSA and providing practical recommendations that allow laboratories to report accurate results regardless of the guidelines they follow.

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Cite This Study

Wang et al. (Mon,) studied this question.

synapsesocial.com/papers/69c37c33b34aaaeb1a67f02f https://doi.org/https://doi.org/10.1128/jcm.00125-26

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