The Novel HLA Class II Allele, HLA‐DPA1*02:167 , Identified by PacBio Sequencing

Key Points

• The aim is to identify novel alleles within the HLA-DPA1 gene using advanced sequencing techniques.
• Utilized PacBio sequencing technology.
• Compared the new allele with the existing HLA-DPA1*02:10 allele.
• Focused on non-synonymous nucleotide substitutions in exon 3.
• Discovered HLA-DPA1*02:167 allele differing from HLA-DPA1*02:10 by one nucleotide substitution.
• Identified the specific change occurring at codon 96.