16S rRNA amplicon sequencing of cecal contents from Hy-Line Brown laying hens

Key Points

• To assess the impact of Limosilactobacillus reuteri on the cecal microbiota of Hy-Line Brown laying hens using 16S rRNA sequencing.
• Collected cecal contents from 42-day-old Hy-Line Brown laying hens
• Utilized 16S rRNA metagenomic sequencing to analyze microbial composition
• Compared microbiota profiles before and after Limosilactobacillus reuteri addition
• Alterations in cecal microbiota composition were observed
• Increased diversity in microbial populations after supplementation
• Specific changes in dominant bacterial species were noted