What question did this study set out to answer?

The aim is to develop a clinical blood test that quantifies monocyte-specific gene expression to improve infection diagnostics.

April 3, 2026Expert Review of Molecular Diagnostics2 citations

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Translation of scRNA-Seq to a clinical blood test for infection diagnostics

NTNelson L.S. TangChinese University of Hong Kong TKTony KwanThe University of Sydney JHJinghan HuangChinese University of Hong Kong

Key Points

The aim is to develop a clinical blood test that quantifies monocyte-specific gene expression to improve infection diagnostics.
Implemented DIRECT LS-TA for analyzing monocyte gene expression.
Utilized quantitative PCR and digital PCR for results.
Compared findings with traditional diagnostic methods.
Gene expression measurements strongly correlate with gold-standard diagnostic methods.
DIRECT LS-TA is more affordable than traditional scRNA-seq techniques.
The method can be conveniently used in clinical laboratories.

Abstract

Traditional diagnostic test for differentiating infection has several limitations requiring breakthrough including turn-around time and cost. DIRECT LS-TA provides a reliable way to quantify monocyte-specific gene expression that strongly correlates with gold-standard methods. It is more affordable than single-cell RNA sequencing and can be readily implemented in clinical laboratories using widely available quantitative PCR or digital PCR machines.

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Translation of scRNA-Seq to a clinical blood test for infection diagnostics

Key Points

Abstract

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