A single 115 kDa isoform of obscurin was identified in the cardiac and skeletal muscles of long-tailed ground squirrels, with no giant isoforms or seasonal changes detected.
A 115 kDa low-molecular-weight isoform of obscurin was identified in the striated muscles of the long-tailed ground squirrel, with no seasonal variations observed.
Absolute Event Rate: 0% vs 0%
Polyclonal antibodies specific to the N-sequence (76–289 aa) of obscurin of the long-tailed ground squirrel (Urocitellus undulatus) were used to study the isoform composition of this protein in two striated muscles: the myocardium of the left ventricle and M. gastrocnemius medialis. One low-molecular-weight isoform of obscurin with a molecular weight of 115 kDa has been identified. No other isoforms of obscurin, including giant A- and B-isoforms, have been detected. Seasonal changes in the levels of the low-molecular-weight obscurin isoform visualized in the cardiac and skeletal muscles of long-tailed ground squirrels were not observed. Further research could focus on sequencing the obscn gene of long-tailed ground squirrels and analyzing obscurin transcripts to obtain antibodies specific to the giant isoforms of this protein.
Uryupina et al. (Mon,) reported a other. A single 115 kDa isoform of obscurin was identified in the cardiac and skeletal muscles of long-tailed ground squirrels, with no giant isoforms or seasonal changes detected.