Examining pre-analytical factors for bioanalysis of protein therapeutics crossing the blood–brain barrier through receptor-mediated transcytosis

Key Points

• The aim is to investigate the influence of pre-analytical factors on the bioanalysis of protein therapeutics that cross the blood-brain barrier.
• Examine various pre-analytical procedures
• Assess their impact on bioanalysis outcomes
• Develop optimized workflow for screening
• Highlighted significant effects of pre-analytical factors on bioanalysis
• Identified specific procedures that could improve efficiency
• Facilitated the development of a more streamlined testing process