The study was conducted to select and optimise a set of markers for highly polymorphic SSR loci for the genetic identification of soybean varieties, identify their biotypes within polymorphic varieties, and evaluate their use in supporting the breeding process during the identification of true F1 hybrids. A panel of 51 accessions and cultivars, as well as 55 hybrid combinations from two rounds of hybridisation, was studied. Identification of parental varieties and analysis of hybrid combinations were performed using the developed set of 11 SSR markers, using multiplex PCR followed by visualisation by capillary electrophoresis. Testing the marker set on a collection of 51 varieties allowed us to distinguish between all genotypes and obtain unique genetic profiles for each accession. The most polymorphic loci in the collection were Satt009 (PIC = 0.79; Na = 8), Satt245 (PIC = 0.73; Na = 5), Satt141 (PIC = 0.7; Na = 6), Satt681 (PIC = 0.68; Na = 8), Satt440 (PIC = 0.67; Na = 5) and Sat177 (PIC = 0.67; Na = 5). Validation of the kit on 55 hybrid combinations also showed high efficiency of hybridity assessment. The most convenient markers for hybrid detection were Satt440, Satt009 and Satt245, well visualised both by fragment analysis and using gel electrophoresis in agarose gel. The markers Satt440, Satt009 and Satt245 distinguished 78% (40 parental pairs), 70% (35 parental pairs), and 75% (38 parental pairs) of all crosses, respectively. The presented approach can be applied in a stepwise manner both for identifying F1 soybean hybrids, primarily at the Satt440, Satt009, and Satt245 loci, and for genetic identification of varieties using the full panel of developed SSR markers.
Klimushina et al. (Wed,) studied this question.
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