What question did this study set out to answer?

The study aims to explore how the tumor microenvironment influences the specialization of cancer cell clones in prostate cancer.

April 5, 2026

Abstract 6233: Proximity-based gene expression profiling identifies multi-pathway activation in perivascular, perineural, and pseudo-capsule adjacent human prostate cancer cells

Key Points

The study aims to explore how the tumor microenvironment influences the specialization of cancer cell clones in prostate cancer.
Employed spatial transcriptomics to profile cancer cell populations adjacent to critical invasive fronts
Analyzed 55 human prostate tissue datasets for differential gene expression
Identified functional signatures specific to niches near nerves, vessels, and the pseudo-capsule
Neurotropic clones near nerves exhibited altered gene expression linked to perineural invasion
Perivascular clones showed signs of extracellular matrix remodeling and an EMT-like state
Clones near the pseudo-capsule displayed a highly proliferative phenotype indicative of aggressive cancer behavior

Abstract

Abstract Prostate cancer (PCa) progression is critically driven by the tumor microenvironment (TME), which imposes selective pressures and dictates specialized invasive behaviors, such as perineural and lymphovascular invasion. We hypothesized that heterogeneity within the tumor allows for the selection of functionally specialized cancer cell clones based on their proximity to TME niches. Using Spatial Transcriptomics, we profiled cancer cell populations at three critical invasive fronts: near nerves, vessels, and the prostatic pseudo-capsule from 55 human prostate tissue datasets. Differential expression analysis revealed distinct, niche-specific functional signatures: neurotropic clones near nerves exhibited a loss of prostate identity and a gain of neural adhesion genes (e.g., NCAM1, NTN1) consistent with perineural invasion; perivascular clones showed upregulation of ECM remodeling and an EMT-like state (e.g., ANXA2, CCN1), suggesting priming for intravasation; and pseudo-capsule-adjacent clones displayed a highly proliferative phenotype (e.g., CCND1, CDK4). These results demonstrate that the TME drives the functional specialization of PCa cells, refining our understanding of invasion and nominating several niche-specific pathways as potential therapeutic targets. Studies are currently ongoing to validate these signatures, refine heterogeneity scoring, and cross-link the identified genes with pharmacological databases for future in-vitro inhibition assays. Citation Format: Rafael Sainz, Kelvin W. Pond, Beatrice S. Knudsen, Gregory C. Rogers, Noel E. Warfel, Anne E. Cress. Proximity-based gene expression profiling identifies multi-pathway activation in perivascular, perineural, and pseudo-capsule adjacent human prostate cancer cells abstract. In: Proceedings of the American Association for Cancer Research Annual Meeting 2026; Part 1 (Regular Abstracts); 2026 Apr 17-22; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2026;86(7 Suppl):Abstract nr 6233.

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