Fluorescence Detection of Intron Lariat RNA with Reduction‐Triggered Fluorescent Probes

Fluorescence through proximity: Reduction-triggered fluorescent probes bearing either a fluorescein derivative or triphenylphosphine bind sequence-specifically at the branch site of lariat RNA. The chemical reaction between the two substrates leads to the formation of unquenched fluorescein and the emission of a fluorescence signal (see picture). Prelariat and lariat structures can be distinguished by using this method. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.