A crucial method for evaluating the potential harm to the genome caused by contaminants at levels exceeding the optimal threshold is the chromosomal plant assay. This paper reports on a study that examined the effects of varying concentrations of manganese (Mn) on the mitotic index (MI), cell kinetics index (CKI), and abnormality index (AI) in Glycine max L. root tip cells. Percentage of mitotic index, abnormality index, cell kinetics index, in root meristems of Glycine max L. at control and varying concentrations of Mn were evaluated. The findings showed that Mn doses that were utilized for seed treatment caused distinct differences in chromosomal activity of Glycine max L. root tip cells, with a decreased mitotic index and cell kinetics, and an increased abnormality index. Treatment was conducted at room temperature for 24 hours, 48 hours, and 72 hours at four different concentrations of Mn: CN (Control), 5μM, 10μM, 15μM, and 20μM. The control group was treated with distilled water. The findings demonstrated that Mn has cytotoxic and genotoxic effects on Glycine max L. root tip cells.
Sazada Siddiqui (Wed,) studied this question.
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