Colorimetric LAMP assay enables rapid detection of Hb Lepore and Hb Sicilian deletions in the β-globin gene cluster with minimal instrumentation

Key Points

• This study aims to develop a rapid and simple colorimetric assay for detecting specific hemoglobin deletions.
• Developed a colorimetric LAMP assay for hemoglobin deletions.
• Assessed visual detection capabilities.
• Evaluated assay performance compared to PCR methods.
• Successfully detected Hb Lepore and Hb Sicilian deletions.
• Demonstrated minimal equipment needs for implementation of the assay.
• Indicated potential use in low-resource or point-of-care settings.