In this study, microscale pattern was fabricated on parafilm and poly (methyl methacrylate) towards efficient bioanalysis of DOX in alkaline solution. Silver nanoprisms (AgNPrs) was utilized as specific optical sensing component for the colorimetric detection of DOX at pH of 9 as optimum pH in the linear concentration range of 5 μM to 40 mM. The electrostatic interaction between DOX and AgNPrs was confirmed through zeta potential (Z p ) analysis after the amalgamation of the optical probe with the analyte. The interaction between AgNPrs, acting as the optical nanoprobe, and DOX, as the analyte, was verified by the change in Z p from −13.6 to −6.18 mV. Subsequently, two innovative micro-arrays were constructed using parafilm and PMMA as suitable substrates, which were used for the one-drop colorimetric chemosensing of DOX, leveraging smartphone-assisted digital image analysis through a Color Picker application (version 7.9.0) that installed on mobile devices. For the first time, two novel micro-scale portable colorimetric substrates (μPCSs) have been introduced, enabling the cost-effective, rapid, and on-site assessment of DOX (as the model analyte) with a low limit of quantification of 50 μM in standard samples and 5 μM in human plasma samples. This study introduces two novel μPCSs based on Parafilm and PMMA, which enable smartphone-assisted in-situ detection of DOX in human blood plasma.
Behyar et al. (Wed,) studied this question.