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This study aims to evaluate the efficacy of mutation-agnostic multiplex drop-off ddPCR assays for monitoring circulating tumor DNA in uveal melanoma.

May 7, 2026Analytical Chemistry0 citationsOpen Access

Circulating Tumor DNA Monitoring in Patients with Uveal Melanoma Using Mutation-Agnostic Multiplex Drop-Off ddPCR Assays

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ARAurore RampanouCouncil of Independent Colleges MRManuel RodriguesCentre National de la Recherche Scientifique FBFrançois-Clément BidardCouncil of Independent Colleges

Key Points

This study aims to evaluate the efficacy of mutation-agnostic multiplex drop-off ddPCR assays for monitoring circulating tumor DNA in uveal melanoma.
Utilized multiplex drop-off ddPCR assays for ctDNA monitoring.
Compared assays with traditional targeted NGS and simplex ddPCR methods.
Emphasized the cost-effectiveness and sensitivity of the multiplex approach.
Multiplex ddPCR assays showed high sensitivity and specificity for ctDNA detection.
Results indicated significant advantages over targeted NGS in clinical settings.
Assays enabled real-time treatment monitoring without prior tumor genotype knowledge.

Abstract

= 11), further supporting the accuracy of the multiplex assays. Our mutation-agnostic multiplex drop-off ddPCR assays provide a sensitive, specific, and cost-effective alternative to targeted NGS and simplex ddPCR for ctDNA monitoring in UM. By minimizing reliance on prior knowledge of tumor genotype with NGS, these assays enable broader clinical applicability for real-time treatment monitoring in UM.

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Circulating Tumor DNA Monitoring in Patients with Uveal Melanoma Using Mutation-Agnostic Multiplex Drop-Off ddPCR Assays

Key Points

Abstract

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