Study the Expression of miRNA let 7 Gene Among Iraqi Breast Cancer Patients

Abstract Background: MicroRNAs (miRNAs) are short non-coding RNA sequences (approximately 22 nucleotides) that play an important role in gene regulation. Objective: The aim of this study was to evaluate the expression of serum (miRlet-7) in the Iraqi pupation as important biomarkers among the Iraqi women. Materials and Methods: This study included 50 female breast cancer patients. Patient’s peripheral blood was obtained, and apparently healthy female volunteers acted as controls. Each individual had 5 mL of venous blood draw under aseptic circumstances, which was directly placed in Trizol preservation for RNA extraction. Circulating serum miRNAlet-7 expression was measured using RT-qPCR. Result: There were significant increases in groups (41–50) years old ( P = 0.016) and over 60 years old ( P = 0.02) compared to control groups. Of these 50 female patients with breast cancer, 28 (46%) were under 50 years old and 22 (44%) were over 50 years old. Approximately 37 (74%) of these hadestrogen-positive receptors (ER) and 36 (72%) had progesterone-positive receptors (PR). Also 20 of 50 cases (20%) had positive and 30 (60%) had HER-2 expression. According to histopathological type 96% were invasive ductal carcinoma (IDC), and 4% were invasive lobular carcinoma (ILC) of all breast cancer patients. Conclusion: Based on the results of this investigation, we find that miRlet-7 expression is significantly higher ( P = 0.003) in breast cancer patients than in apparently healthy controls; therefore, it is correlated with breast cancer. Also, this study found that among Iraqi women with breast cancer, the occurrence of the Ductal subtype is more significant than that of the lobular subtype.