This study aimed to investigate a four-month Ralstonia pickettii outbreak by characterizing clinical and environmental isolates while also demonstrating the infection control measures taken to identify and eliminate the sources.R. pickettii was isolated from patients' blood cultures, and an epidemiological investigation was initiated to determine the source of the outbreak.Clonal relatedness among clinical and environmental samples was assessed using PFGE.Antimicrobial susceptibility profiles were determined through the disk diffusion method.In total, R. pickettii was isolated from 14 patient blood cultures and three bottles of sterile distilled water.Between April 14 and May 15, six patients had positive port cultures, including four with positive peripheral cultures.The contaminated water was identified as the outbreak source, and its use was discontinued on May 15.Nevertheless, two additional cases occurred in June.Port-catheter colonization with R. pickettii was considered to be linked to contaminated water used during chemotherapy preparation.A recall of port-catheter patients who received chemotherapy in April-May identified six more cases.PFGE revealed two pulsotypes among water isolates, and most clinical isolates were clonally related to Clone 1.The strains were generally susceptible to the tested antimicrobials.This investigation highlights the role of contaminated sterile distilled water and colonized medical devices as potential sources of R. pickettii outbreaks.
Alp et al. (Thu,) studied this question.