Rotavirus serotype 1 was predominant in the USA from 1987-1989, and PCR amplification successfully typed the 17% of rotavirus-positive specimens that were untypable by standard serologic techniques.
Observational (n=281)
Yes
Serotype 1 was the predominant rotavirus strain in the USA from 1987-1989, and PCR typing proved highly valuable for identifying strains that were untypable by standard serologic methods.
The epidemiology of rotavirus gastroenteritis was investigated for two consecutive seasons (1987-1988 and 1988-1989) in seven locales in the continental USA. The 281 representative fecal samples obtained from children with diarrhea were electropherotyped and serotyped by an enzyme immunoassay with serotype-specific monoclonal antibodies and a new amplification typing technique (polymerase chain reaction typing). Serotype 1 was predominant in both years, particularly in the North and East; serotype 3 was second in frequency and found most often in the South; serotype 2 was detected only occasionally; serotypes 4, 8, and 9 were never found. Rotavirus strains were grouped into five major electropherotypes, each corresponded to a single serotype, and the relative migration of the gene segments 7-9 could be used to distinguish serotype 1 from serotype 3. The amplification typing technique proved to be of great value in typing the 17% of rotavirus-positive specimens untypable by the serologic technique.
Gouvêa et al. (Wed,) conducted a observational in Rotavirus gastroenteritis (n=281). Electropherotyping and serotyping (EIA and PCR) was evaluated on Serotype and electropherotype distribution. Rotavirus serotype 1 was predominant in the USA from 1987-1989, and PCR amplification successfully typed the 17% of rotavirus-positive specimens that were untypable by standard serologic techniques.
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