Catalytic and regulatory basis of tRNA t6A modification by the KEOPS complex

Key Points

• The aim is to elucidate the catalytic and regulatory mechanisms of tRNA t6A modification by the KEOPS complex.
• Structural analysis of the KEOPS-tRNA complex
• Assessment of GAMOS-associated mutations
• Identification of functional hotspots within the complex.
• The KEOPS complex exhibits specific structural features crucial for tRNA modification.
• GAMOS-associated mutations identified at functional hotspots affect the efficiency of modification.
• Findings provide new insight into the biochemical roles of the KEOPS complex in cellular function.