Overexpression of human profilin in clonal cell lines increased filamentous actin concentration and half-life, and altered actin distribution by increasing submembranous actin network density.
In vivo profilin regulates the stability and distribution of specific dynamic actin structures.
We describe the production and analysis of clonal cell lines in which we have overexpressed human profilin, a small ubiquitous actin monomer binding protein, to assess the role of profilin on actin function in vivo. The concentration of filamentous actin is increased in cells with higher profilin levels, and actin filament half-life measured in these cells is directly proportional to the steady-state profilin concentration. The distribution of actin filaments is altered by profilin overexpression. While parallel actin bundles crossing the cells are virtually absent in cells overexpressing profilin, the submembranous actin network of these cells is denser than in control cells. These results suggest that in vivo profilin regulates the stability, and thereby distribution, of specific dynamic actin structures.
Finkel et al. (Tue,) reported a other. Overexpression of human profilin vs. Control cells was evaluated on Actin filament concentration, half-life, and distribution. Overexpression of human profilin in clonal cell lines increased filamentous actin concentration and half-life, and altered actin distribution by increasing submembranous actin network density.