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Abstract A simplified procedure for purification of terminal deoxynucleotidyl transferase is presented. The enzyme is shown to be homogeneous by equilibrium centrifugation and gel electrophoresis. The molecular weight of the enzyme is 32,360, with a v measured as 0.65 cc g-1. The homogeneous enzyme can be dissociated into two subunits, α and β, by sodium dodecyl sulfate. Subunit molecular weights, estimated from gel electrophoresis, are α = 8,000 and β = 26,500.
Chang et al. (Mon,) studied this question.