Elevated expression of KLRG1 on CD8+ Tcm cells (HR 1.003) and PD-1 on CD8+ Tnaive cells (HR 1.002) were identified as independent risk factors for atrial fibrillation recurrence after cryoballoon ablation.
Cohort (n=85)
No
Are exhausted T cell markers associated with atrial fibrillation recurrence after cryoballoon ablation?
T cell exhaustion markers, specifically KLRG1 of CD8+ Tcm and PD-1 of CD8+ Tnaive, are independent predictors of AF recurrence after cryoballoon ablation and can be incorporated into a predictive nomogram.
Effect estimate: HR 1.003 (95% CI 1.001-1.006)
p-value: p=0.0063
Background Atrial fibrillation (AF) recurrence remains a challenge after Cryoballoon ablation (CBA). To date, few studies have investigated the impact of immunosenescence, particularly exhausted T cells, on AF recurrence after CBA. This study aims to investigate the association between exhausted T cells and AF recurrence after CBA, and to develop a novel model for the prediction of AF recurrence. Methods Clinical data of patients undergoing CBA treatment for AF at Tianjin Medical University Second Hospital from March to November 2022 were collected, and AF recurrence was followed up. Flow cytometry was employed to evaluate the levels of inhibitory receptors (IR) on exhausted T cells, including killer cell lectin-like receptor G1 (KLRG1), programmed death-1 (PD-1), T cell immunoglobulin and mucin domain-containing molecule 3 (Tim-3), lymphocyte activation gene 3 (LAG-3), CD27, and CD57. Variables selected through Lasso regression analyses were incorporated into a multivariable Cox proportional hazards model to validate and identify independent risk factors for AF recurrence, building two predictive models. The efficacy of the two models was assessed using Receiver Operating Characteristic (ROC) curves, calibration curves, and Decision Curve Analysis (DCA). Results Cox multivariate regression analysis revealed that KLRG1 of CD8 + T central memory cell (Tcm), PD-1 of CD8 + naive T cell (Tnaive), mean corpuscular volume (MCV), γ-Glutamyl Transferase (γ-GGT), glucose, and mitral valve maximum blood flow velocity (MV Vmax) were significant predictors. Two models were developed based on Cox multivariate regression analysis. Model 1 includes MCV, γ-GGT, glucose, and MV Vmax, while Model 2 includes all risk factors: MCV, γ-GGT, glucose, MV Vmax, KLRG1 of CD8 + Tcm, and PD-1 of CD8 + Tnaive. Validation of both models revealed that Model 2 showed better predictive performance, and a nomogram was created to present the results visually. Conclusion The KLRG1 of CD8 + Tcm and PD-1 of CD8 + Tnaive are novel independent risk factors for AF recurrence after CBA and play a significant role in the early recurrence of AF. We constructed a new predictive nomogram incorporating KLRG1 of CD8 + Tcm and PD-1 of CD8 + Tnaive as key variables, which can enhance the predictive value for AF recurrence in patients after CBA surgery.
Suo et al. (Tue,) conducted a cohort in Atrial fibrillation (n=85). Cryoballoon ablation was evaluated on Atrial fibrillation recurrence (HR 1.003, 95% CI 1.001-1.006, p=0.0063). Elevated expression of KLRG1 on CD8+ Tcm cells (HR 1.003) and PD-1 on CD8+ Tnaive cells (HR 1.002) were identified as independent risk factors for atrial fibrillation recurrence after cryoballoon ablation.