Angiotensin II infusion provoked similar increases in blood pressure, aldosterone, distal transporters, ENaC channel activation, and potassium loss in both female and male rats and mice.
Does angiotensin II-induced hypertension provoke similar electrolyte and transporter responses in female and male rats and mice?
Despite baseline sexual dimorphisms, angiotensin II-induced hypertension provokes similar increases in blood pressure, aldosterone, and distal sodium transporter activation in both female and male rodents.
Abstract Aim Sexual dimorphisms are evident along the nephron: Females (F) exhibit higher ratios of renal distal to proximal Na + transporters' abundance, greater lithium clearance (C Li ) more rapid natriuresis in response to saline infusion and lower plasma K + vs. males (M). During angiotensin II infusion hypertension (AngII‐HTN) M exhibit distal Na + transporter activation, lower proximal and medullary loop transporters, blunted natriuresis in response to saline load, and reduced plasma K + . This study aimed to determine whether responses of F to AngII‐HTN mimicked those in M or were impacted by sexual dimorphisms evident at baseline. Methods Sprague Dawley rats and C57BL/6 mice were AngII infused via osmotic minipumps 2 and 3 weeks, respectively, and assessed by metabolic cage collections, tail‐cuff sphygmomanometer, semi‐quantitative immunoblotting of kidney and patch‐clamp electrophysiology. Results In F rats, AngII‐infusion increased BP to 190 mm Hg, increased phosphorylation of cortical NKCC2, NCC and cleavage of ENaC two to threefold, increased ENaC channel activity threefold and aldosterone 10‐fold. K + excretion increased and plasma K + decreased. Evidence of natriuresis in F included increased urine Na + excretion and C Li , and decreased medullary NHE3, NKCC2 and Na,K‐ATPase abundance. In C57BL/6 mice, AngII‐HTN increased abundance of distal Na + transporters, suppressed proximal‐medullary transporters and reduced plasma K + in both F and M. Conclusion Despite baseline sexual dimorphisms, AngII‐HTN provokes similar increases in BP, aldosterone, distal transporters, ENaC channel activation and K + loss accompanied by similar suppression of proximal and loop Na + transporters, natriuresis and diuresis in females and males.
Veiras et al. (Wed,) conducted a other in Angiotensin II-induced hypertension. Angiotensin II infusion vs. Males (for sex comparison) was evaluated on Blood pressure, aldosterone, distal transporters, ENaC channel activation, and K+ loss. Angiotensin II infusion provoked similar increases in blood pressure, aldosterone, distal transporters, ENaC channel activation, and potassium loss in both female and male rats and mice.
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