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June 1, 2026Open Access

Glycyrrhiza uralensis-Atractylodes macrocephala combination supplementation enhances broiler growth performance, immunity, and intestinal health, potentially mediated through gut microbiota and metabolites

Key Points

This study aims to evaluate the effects of Glycyrrhiza uralensis - Atractylodes macrocephala combination supplementation on broiler growth, immunity, and intestinal health.
Broilers were randomly assigned to control and treatment groups with different GA supplementation levels (0.1% and 0.3%).
The trial lasted for 84 days, assessing growth performance, meat quality, and immune responses.
Data on gut microbiota composition and metabolomic analysis were collected to assess intestinal health.
The high GA supplementation (HGA) notably improved average daily gain (ADG) and reduced feed-to-gain ratio (F/G).
HGA enhanced immune function, increasing IgA, IgM, and IL-10 levels while decreasing IL-2 and TNF-α.
Alterations in gut microbiota with increased beneficial bacteria and metabolites were observed, indicating improvements in intestinal health.

Abstract

The poultry industry requires effective antibiotic alternatives to enhance growth and maintain health. This study investigated the effects of the Glycyrrhiza uralensis - Atractylodes macrocephala combination (GA) on growth, meat quality, and health in broilers. One-day-old male Lingnan yellow broilers were randomly assigned to a control group (fed a basal diet) and two treatment groups that received diets supplemented with 0. 1% (LGA) and 0. 3% (HGA) GA, respectively. The experiment lasted for 84 days. The results showed that HGA significantly enhanced average daily gain (ADG) in broilers aged 29–56, 57–84, and 1–84 days, accompanied by a decreased feed-to-gain ratio (F/G). HGA increased carcass performance by increasing leg muscle rate and decreasing abdominal fat rate at day 84, and improved meat quality by reducing L* and b* values and shear force, and increasing a* value in breast muscle. HGA elevated the thymus and bursa of Fabricius indices and serum immunoglobulin A (IgA), immunoglobulin M (IgM), and interleukin-10 (IL-10) levels, while decreasing interleukin-2 (IL-2) and tumor necrosis factor-α (TNF-α) levels. Moreover, HGA increased jejunal villus height (VH) and villus height/crypt depth (VH/CD), and secretory immunoglobulin A (sIgA) content and mRNA expression of ZO-1 and CLDN1. It also modulated the cecal microbiota composition and altered microbial interactions in an age-dependent way. Specifically, HGA increased the abundance of short-chain fatty acids-producing or anti-inflammatory-associated bacteria, such as RikenellaceaeRC9gutgroup, Negativibacillus, Enterococcus, Butyricicoccus, and Muribaculaceae, while decreasing the abundance of pro-inflammatory-associated bacteria, such as Parasutterella, Desulfovibrio, and Campylobacter. Metabolomic analysis revealed that HGA altered cecal metabolic profiles by upregulating key metabolites, including 3α, 7α, 12α-trihydroxy-5β-cholestanoate, enoxolone, and butyric acid. In conclusion, HGA enhances production performance, immune function, and intestinal barrier integrity in broilers, where shifts in gut microbiota and metabolites may contribute to these beneficial outcomes. These findings provide a solid scientific basis for the design and utilization of herbal feed additives.

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Cite This Study

Tian et al. (Thu,) studied this question.

synapsesocial.com/papers/6a1d20bc02fbce9130637060 https://doi.org/https://doi.org/10.3389/fvets.2026.1855302

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