Application of a three-phase fluidized bed electrode for an electro-enzymatic batch process with the peroxygenase AaeUPO

Key Points

• This study aims to explore the use of a fluidized bed electrode in enhancing the electro-enzymatic process for hydroxylation reactions.
• First utilization of a fluidized bed electrode for an electro-enzymatic process.
• In-situ generation of co-substrates for AaeUPO's reaction.
• Optimization of H₂O₂ rate and enzyme concentration to improve reaction outcomes.
• Successfully hydroxylated 4-ethylbenzoic acid to 4-(1-hydroxyethyl)benzoic acid with improved efficiency.
• Higher current efficiency and yield achieved through enzyme and co-substrate tuning.