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Absorption spectra of several phytoplankton species were decomposed, after correction for the particle effect, to estimate in vivo absorption properties of the major light-harvesting pigments in algae.A Gaussian shape is suitable, theoretically and empirically, to represent the absorption spectra of individual photosynthetic components.The Gaussian parameters agreed well with the expected pigment compositions of 3 groups of algae, and the peak heights were linearly correlated with the concentrations of any one of the 4 major pigments measured in the samples.The linear relationship did not vary with phytoplankton species.We present here the first estimates of the 'true' in vivo specific absorption coefficients of 4 major pigments, after correction for particle effect.The results are used to reconstruct the in vivo absorption spectrum of a multi-species sample.
Hoepffner et al. (Tue,) studied this question.