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had been processed within 30 minutes after resection. Similar proportions of benign and malignant tissue grew facultative bacterial species. There were delays of 16 and 20 hours in processing two malignant samples; neither of these grew anaerobes. The anaerobes isolated included anaerobic cocci (four samples), Bacteroides distasonis (one), and Clostridium perfringens (one). Anaerobes were isolated from only one specimen before broth enrichment.
Johnstone et al. (Sat,) studied this question.
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