Sphingomyelinase and cell-permeable ceramide analogs stimulate cellular proliferation in quiescent Swiss 3T3 fibroblasts.

Sphingomyelin Turnover and Cellular Proliferation chloride (79.3 Ci/mmol), and y-3'PATP (3000 Ci/mmol) were purchased from Amersham Corp. Insulin and transferrin were from Collaborative Research (Lexington, MA).Sphingomyelinase (from brain, type HI), and DETAPAC' were from Sigma.Diacylglycerol Streptomyces sp. and Staphylococcus aureus), ceramides (from bovine kinase (from Escherichia coli) was from Lipidex, Inc. (Westfield, NJ).The various standard phospholipids and cardiolipin were from Avanti Polar Lipids.N-Hexanoylsphingosine