Soybean (Glycine max (L.) Merr.) is a key legume with high agronomic and nutritional value, widely cultivated for its high-protein seeds and its capability to improve soil fertility through biological nitrogen fixation. Recently, co-inoculation strategies combining Rhizobia bacteria with growth-enhancing fungi from the genus Trichoderma have gained increasing attention as a way to enhance soybean productivity and resilience under variable environmental conditions. The present study, conducted in 2023–2024, examined how seed inoculation treatments (Bradyrhizobium japonicum and Trichoderma viride) affect soybean productivity, seed quality, and soil biochemical changes expressed as the activity levels of selected soil enzymes—dehydrogenases (DHA), catalase (CAT), acid phosphatase (ACP), and alkaline phosphatase (ALP)—as well as the biological fertility index (BIF). The findings indicated that co-inoculation positively influenced plant productivity and produced the highest seed yields among all treatments tested, exceeding the control by 25.6%. Furthermore, inoculated seeds were characterized by improved seed quality, expressed by higher germination capacity (80%) and greater average seedling length (4.74 cm). The bacterial strains used to inoculate soybean seeds increased soil biochemical activity and improved fertility, particularly under unfavorable rainfall distribution during the growing season. Co-inoculation can be recommended as an effective and environmentally friendly element of soybean cultivation technology, supporting yield stability in variable weather conditions. Further research is recommended in longer multi-year series and under various habitat conditions.
Panasiewicz et al. (Fri,) studied this question.