Characterisation of the Novel HLA‐DQA1*05:65:01:01 Allele by Next‐Generation Sequencing

Key Points

• To characterize the novel HLA-DQA1*05:65:01:01 allele and understand its genetic differences.
• Used next-generation sequencing to analyse the HLA-DQA1 gene.
• Identified nucleotide substitutions in the allele.
• Focused on codon 218 in exon 4 for genetic differences.
• HLA-DQA1*05:65:01:01 differs from HLA-DQA1*05:05:01:02 by one nucleotide substitution.
• The specific substitution was identified at codon 218 in exon 4.