Dual-readout aptasensor based on CRISPR/Cas12a and nanozyme for accurate detection of KIM-1 and its application in kidney transplant prognosis

Key Points

• Significant accuracy in KIM-1 detection supports better kidney transplant prognosis.
• The aptasensor demonstrated a sensitivity of 0.5 ng/mL for KIM-1, enhancing early detection abilities.
• Analysis combines the CRISPR/Cas12a system with a nanozyme approach for effective diagnostics.
• This work provides a foundation for advanced kidney health monitoring; further clinical validation is encouraged.