Novel Turn-on Fluorescent Probe for Endogenous Peroxynitrite Detection Based on Selective Oxidation of Nitrobenzoyl Formate Ester

Key Points

• This research aims to develop a new fluorescent probe for the specific detection of endogenous peroxynitrite.
• Designed a turn-on fluorescent probe (EASHC-NO2) for detection.
• Utilized intramolecular charge transfer (ICT) mechanism.
• Focused on selective oxidation of nitrobenzoyl formate ester for sensing.
• Successfully demonstrated fluorescence activation upon peroxynitrite presence.
• Proved effective detection of peroxynitrite in biological samples.
• Showed high selectivity and sensitivity towards peroxynitrite.