What question did this study set out to answer?

The aim is to establish and optimize a multicolour flow cytometry panel for identifying human amniotic fluid stem cells.

April 22, 2026Open Access

A multicolour flow cytometry panel to characterize human amniotic fluid stem cells

Key Points

The aim is to establish and optimize a multicolour flow cytometry panel for identifying human amniotic fluid stem cells.
Utilized a 16 colour flow cytometry panel for characterization.
Optimized titration and fixation/permeabilization for each antibody.
Included multiple markers such as CD29, CD34, and OCT3/4.
Successfully established a functional multicolour panel.
Demonstrated effective characterization of stem cell markers.
Enhanced identification of amniotic fluid stem cells using the optimized panel.

Abstract

Establishment and optimization of a multicolour flow cytometry panel to characterize human amniotic fluid stem cells. Titration and fixation/permeabilization optimization of each antibody used in this 16 colour panel. Markers used: CD29, CD34, CD44, CD45, CD73, CD90, CD105, CD117, CD133, HLA-ABC, HLA-DR, OCT3/4, SSEA-3, SSEA-4, TRA-1-60, TRA-1-81 and live/dead discriminator.

A multicolour flow cytometry panel to characterize human amniotic fluid stem cells

Key Points

Abstract

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