Design of 365 nm-Light-triggered Fluorogenic Crosslinking via a Genetically Encoded 3-CF 3 - p -phenylenediamine-Lysine Featuring Red-shifted Photo-defluorination

Key Points

• To develop and characterize a new genetically encodable non-canonical amino acid (ncAA) that improves photo-defluorination efficiency for crosslinking applications.
• Introduced 3-CF3-p-phenylenediamine-L-lysine as a genetically encodable ncAA.
• Evaluated the red-shifted activation at 365 nm for photo-defluorination.
• Compared the efficiency of the new ncAA against first-generation versions.
• Achieved faster photo-defluorination with the new ncAA compared to the first-generation versions.
• Demonstrated successful crosslinking in a light-triggered setup, indicating enhanced performance.
• Establishes a base for future applications of light-activated genetic tools.