Design of 365 nm-Light-triggered Fluorogenic Crosslinking via a Genetically Encoded 3-CF ₃ - p -phenylenediamine-Lysine Featuring Red-shifted Photo-defluorination

Fan Zhang; Ziming Xiong; Siyu Kuang; Sitong Li; Xiaohu Zhao; Zhipeng Yu

doi:10.1039/d6qo00284f

January 1, 2026Organic Chemistry Frontiers

Design of 365 nm-Light-triggered Fluorogenic Crosslinking via a Genetically Encoded 3-CF ₃ - p -phenylenediamine-Lysine Featuring Red-shifted Photo-defluorination

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Key Points

To develop and characterize a new genetically encodable non-canonical amino acid (ncAA) that improves photo-defluorination efficiency for crosslinking applications.
Introduced 3-CF3-p-phenylenediamine-L-lysine as a genetically encodable ncAA.
Evaluated the red-shifted activation at 365 nm for photo-defluorination.
Compared the efficiency of the new ncAA against first-generation versions.
Achieved faster photo-defluorination with the new ncAA compared to the first-generation versions.
Demonstrated successful crosslinking in a light-triggered setup, indicating enhanced performance.
Establishes a base for future applications of light-activated genetic tools.

Implication

This randomized trial evaluates the efficacy of a novel photo-activated crosslinking approach, suggesting advanced capabilities for genetic applications.

Abstract

We introduce 3-CF 3 - p -phenylenediamine-L-lysine, a genetically encodable photo-Defluorination-Acyl Fluoride Exchange ncAA. It features red-shifted activation (311 → 365 nm) for faster photo-defluorination relative to 1 st generation photo-DAFEx ncAA, and subsequent Ac-F...

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Cite This Study

Zhang et al. (2026) studied this question.

synapsesocial.com/papers/69ec5bd288ba6daa22dad28f https://doi.org/10.1039/d6qo00284f

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Design of 365 nm-Light-triggered Fluorogenic Crosslinking via a Genetically Encoded 3-CF ₃ - p -phenylenediamine-Lysine Featuring Red-shifted Photo-defluorination

Key Points

Implication

Abstract

Discussion

Cite This Study

Design of 365 nm-Light-triggered Fluorogenic Crosslinking via a Genetically Encoded 3-CF ₃ - p -phenylenediamine-Lysine Featuring Red-shifted Photo-defluorination

Key Points

Implication

Abstract

Discussion

Cite This Study

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