Oleuropein derived from olive leaves inhibits the activity of Helicobacter pylori urease: network pharmacology, inhibition kinetics, and mechanistic insights

Key Points

• This research aims to explore how oleuropein affects urease activity in Helicobacter pylori and to shed light on the underlying mechanisms.
• Conducted inhibition kinetics to assess oleuropein's effect on urease activity.
• Performed mechanistic analyses focusing on thiol groups and specific residues of urease.
• Oleuropein inhibited H. pylori urease activity in a time-dependent manner.
• The inhibition was found to be mixed-type and reversible.
• Mechanistic studies indicated involvement of thiol groups and the CYS321 flap residue.