Research on a Highly Sensitive Aptamer Sensor for Vibrio alginolyticus Based on CRISPR-Cas13a and T7 Transcription Cascade Amplification

Key Points

• This research aims to develop a highly sensitive sensor for detecting Vibrio alginolyticus using advanced molecular techniques.
• Utilized an aptamer sensor integrated with CRISPR-Cas13a and T7 transcription cascade amplification.
• Tested the sensor for its sensitivity and specificity in detecting Vibrio alginolyticus.
• Conducted experiments to evaluate detection speed and accuracy.],
• Achieved detection of Vibrio alginolyticus with a sensitivity rate of 95%, p<0.001.
• The sensor demonstrated a detection speed under 30 minutes, providing rapid results for public health applications.
• Specificity of the sensor was confirmed at 97%, ensuring minimal false positives.