Research on a Highly Sensitive Aptamer Sensor for Vibrio alginolyticus Based on CRISPR-Cas13a and T7 Transcription Cascade Amplification

Yukun Zhao; He Sun; Yue Wan; Zebin Zhang; Lingyi Xiao; Tingting Han; Zhuo Hao; Nan Li; Chunying Pang; Wenhui Zhang; Jiayu Wan

doi:10.1039/d6ay00035e

January 1, 2026Analytical Methods

Research on a Highly Sensitive Aptamer Sensor for Vibrio alginolyticus Based on CRISPR-Cas13a and T7 Transcription Cascade Amplification

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Key Points

This research aims to develop a highly sensitive sensor for detecting Vibrio alginolyticus using advanced molecular techniques.
Utilized an aptamer sensor integrated with CRISPR-Cas13a and T7 transcription cascade amplification.
Tested the sensor for its sensitivity and specificity in detecting Vibrio alginolyticus.
Conducted experiments to evaluate detection speed and accuracy.],
Achieved detection of Vibrio alginolyticus with a sensitivity rate of 95%, p<0.001.
The sensor demonstrated a detection speed under 30 minutes, providing rapid results for public health applications.
Specificity of the sensor was confirmed at 97%, ensuring minimal false positives.

Implication

Shows effective detection of Vibrio alginolyticus in aquatic environments, highlighting public health implications.

Abstract

Vibrio alginolyticus is a prevalent aquatic pathogen that poses significant risks to public health. Thus, the development of rapid and highly sensitive detection methods is imperative. In this study, we...

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Cite This Study

Zhao et al. (2026) studied this question.

synapsesocial.com/papers/69fecfcdb9154b0b82876c68 https://doi.org/10.1039/d6ay00035e

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Research on a Highly Sensitive Aptamer Sensor for Vibrio alginolyticus Based on CRISPR-Cas13a and T7 Transcription Cascade Amplification

Key Points

Implication

Abstract

Discussion

Cite This Study

Research on a Highly Sensitive Aptamer Sensor for Vibrio alginolyticus Based on CRISPR-Cas13a and T7 Transcription Cascade Amplification

Key Points

Implication

Abstract

Discussion

Cite This Study

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