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January 1, 1977Nucleic Acids Research716 citationsOpen Access

Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide

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JCJames W. CaseyCornell UniversityNDNorman DavidsonUniversity of California, Riverside

Key Points

  • This study aims to compare the thermal stabilities of RNA:DNA hybrids to DNA:DNA duplexes in high formamide concentrations.
  • Measured the formation rates of RNA:DNA hybrids and DNA:DNA duplexes in aqueous solutions with high formamide concentrations.
  • Assessed thermal stabilities across a range of temperatures.
  • RNA:DNA hybrids show substantially greater thermal stabilities than DNA:DNA duplexes in high formamide.
  • Measured association rates indicate that DNA:DNA rates are negligible while RNA:DNA rates are close to optimal in specific temperature ranges.

Abstract

The thermal stabilities of RNA:DNA hybrids are substantially greater than those of DNA:DNA duplexes in aqueous electrolyte solutions containing high concentrations of formamide. Association rates to form DNA:DNA duplexes and DNA:RNA hybrids have been measured in these solvents. There is a temperature range in which DNA:DNA rates are negligible and RNA:DNA rates close to optimal.

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Cite This Study

Casey et al. (1977) studied this question.

synapsesocial.com/papers/6a09340642764e571715fa15https://doi.org/10.1093/nar/4.5.1539
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