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This is a Experimental Study study.

September 20, 2025

PCR Run Protocol for Bacterial 16S rRNA Gene Amplification v1

MAMohammad AliCentre National de la Recherche Scientifique FMFatima Tuz Zohora Mony JEJ. D. EisenbackSchlumberger (Ireland)

Key Points

High-quality amplification results depend on proper sterile techniques and contamination prevention.
The method utilizes Promega GoTaq® Green Master Mix for bacterial 16S rRNA gene amplification.
The protocol includes negative controls to ensure validity of the PCR reactions.
Standard thermal cycling is optimized specifically for bacterial DNA applications.

Abstract

This protocol describes thepolymerase chain reaction (PCR) setup and amplificationof bacterial16S rRNA genesusing27F and 1492R primers. The method utilizesPromega GoTaq® Green Master Mix (2x)and follows a standardthermal cycling programoptimized for bacterial DNA. Propersterile techniques, contamination prevention, and accurate pipettingare crucial to obtaining high-quality amplification results. The protocol includesnegative controlsto ensure reaction validity.

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