This protocol details PCR setup and amplification of bacterial 16S rRNA genes, ensuring accurate results.
This protocol describes thepolymerase chain reaction (PCR) setup and amplificationof bacterial16S rRNA genesusing27F and 1492R primers. The method utilizesPromega GoTaq® Green Master Mix (2x)and follows a standardthermal cycling programoptimized for bacterial DNA. Propersterile techniques, contamination prevention, and accurate pipettingare crucial to obtaining high-quality amplification results. The protocol includesnegative controlsto ensure reaction validity.
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Ali et al. (2025) studied this question.
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