miR-145-5p combined with miR-140-5p regulates the differentiation of bone marrow mesenchymal stem cells into nucleus pulposus cells and its mechanism in the treatment of intervertebral disc degeneration

Key Points

• This research aims to explore how miR-145-5p and miR-140-5p influence the differentiation of bone marrow mesenchymal stem cells into nucleus pulposus-like cells.
• In vitro analysis of the effects of miR-145-5p and miR-140-5p on BMSC differentiation
• Target identification of miR-145-5p through SOX9
• Assessment of NPC-like phenotypic markers
• miR-145-5p and miR-140-5p synergistically promote BMSC differentiation into NPC-like cells
• SOX9 is identified as a key target of miR-145-5p
• The findings suggest a potential pathway for therapeutic intervention in intervertebral disc degeneration