What question did this study set out to answer?

To determine the prevalence of TEM and OXA beta lactamases in Escherichia coli and identify optimal detection methods.

February 22, 2026

Genomic analysis of TEM and OXA enzymes in clinical isolates of Escherichia coli

Key Points

To determine the prevalence of TEM and OXA beta lactamases in Escherichia coli and identify optimal detection methods.
Analyzed clinical isolates of Escherichia coli for prevalence of beta lactamases.
Assessed minimum inhibitory concentrations for Piperacillin-tazobactam.
Evaluated various detection methods for resistance mechanisms.
Identified significant prevalence of TEM and OXA enzymes in Escherichia coli isolates.
Observed borderline MICs that complicate resistance detection.
Suggests routine susceptibility testing may fail to identify some resistant strains.

Abstract

Introduction: Piperacillin-tazobactam (PTZ) resistance in Escherichia coli is mediated by TEM and OXA enzymes. However, such isolates may have borderline minimum inhibitory concentrations (MICs) between eight (susceptible) and 16 (susceptible but dose dependent) μg/mL. Routine susceptibility testing may fail to detect these mechanisms, leading to clinical failure. We aimed to determine the prevalence of these beta lactamases and the optimal detection method.

Bookmark

Cite This Study

Huang et al. (Sun,) studied this question.

synapsesocial.com/papers/699a534bfdaf4e3c1268eef3 https://doi.org/https://doi.org/10.1016/j.pathol.2026.01.319

Bookmark