CRISPR-Cas12a Amplified RNase Activity Sensor Powered by Gold Nanoparticle-Barcode DNA Multipliers

Key Points

• The aim is to develop a highly sensitive sensor for detecting ribonuclease A activity using CRISPR-Cas12a technology.
• Utilized CRISPR-Cas12a for amplification of signal.
• Incorporated gold nanoparticles and barcode DNA for enhanced sensitivity.
• Evaluated the performance in detecting RNase A levels.
• Achieved rapid detection of RNase A with high sensitivity.
• Demonstrated capability of distinguishing RNase A activity from normal levels.