Abstract Colorectal cancer (CRC) is a frequent gastrointestinal malignancy with high rates of morbidity and mortality. Our previous studies have shown macrophages exhibited significant variations in stage III-IV colon cancer compared to other immune cells. Current study reviewed 11 stage III-IV CRC patient archived FFPE samples carried varied pathology and treatment conditions to investigate macrophage cell features. IHC control, multiplex immunofluorescence (mIF) panel control and the followed mIF staining were conducted using PN 7-Plex Detection Kit (PhenoVision Bio Co., Itd) targeted CD68, CD163, HLA-DR, panCK, SPP1 and PD-L1. The QC procedures made sure each marker from mIF results exhibited identical location and density of the same marker from IHC staining. The 11 staining slides were scanned, tumor areas were lined out by pathologist based on H Part 1 (Regular Abstracts); 2026 Apr 17-22; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2026;86(7 Suppl):Abstract nr 7439.
Xu et al. (Fri,) studied this question.