What question did this study set out to answer?

To investigate the spatial features of SPP1 positive macrophages and their correlation with pathology parameters in stage III-IV colorectal cancer (CRC).

April 5, 2026Cancer Research0 citations

View Full Paper

Abstract 7439: SPP1 positive macrophage spatial feature correlation to stage III-IV colon cancer multiple pathology parameters

SXShaojun XuMinistry of Education HSHongzhe SunInferVision (China)SHShuo HanInferVision (China)

Key Points

To investigate the spatial features of SPP1 positive macrophages and their correlation with pathology parameters in stage III-IV colorectal cancer (CRC).
Reviewed archived FFPE samples from 11 stage III-IV CRC patients.
Conducted IHC and multiplex immunofluorescence staining using a 7-Plex Detection Kit.
Analyzed tumor regions and non-tumor stroma using AI analysis system for positive cell percentage.
Performed statistical analysis to determine correlations with pathology parameters.
Found correlation between CD68+/SPP1+ co-positive cells and PCR/non-PCR with p=0.05.
Identified CD68+/SPP1+ cells' lower percentages in PCR vs. non-PCR (0.46% vs. 2.01%, p=0.049).
More CD68+/SPP1+ cells in pMMR vs. dMMR group (1.94% vs. 0.24%, p=0.027).
PD-L1 positive cell percentages were significantly higher in dMMR vs. pMMR in tumor parenchyma region (10.09% vs. 1.04%, p=0.024).

Abstract

Abstract Colorectal cancer (CRC) is a frequent gastrointestinal malignancy with high rates of morbidity and mortality. Our previous studies have shown macrophages exhibited significant variations in stage III-IV colon cancer compared to other immune cells. Current study reviewed 11 stage III-IV CRC patient archived FFPE samples carried varied pathology and treatment conditions to investigate macrophage cell features. IHC control, multiplex immunofluorescence (mIF) panel control and the followed mIF staining were conducted using PN 7-Plex Detection Kit (PhenoVision Bio Co., Itd) targeted CD68, CD163, HLA-DR, panCK, SPP1 and PD-L1. The QC procedures made sure each marker from mIF results exhibited identical location and density of the same marker from IHC staining. The 11 staining slides were scanned, tumor areas were lined out by pathologist based on H Part 1 (Regular Abstracts); 2026 Apr 17-22; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2026;86(7 Suppl):Abstract nr 7439.

Demander à l'IA

Bookmark

View Full Paper

Demander à l'IA

Bookmark

View Full Paper

Abstract 7439: SPP1 positive macrophage spatial feature correlation to stage III-IV colon cancer multiple pathology parameters

Key Points

Abstract

Cite This Study

Also Consider

Also Consider